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Anti-Human Intra-Cellular Adhesion Molecule 3 (ICAM-3) - 100 µg

Anti-Human Intra-Cellular Adhesion Molecule 3 (ICAM-3) - 100 µg

Item no.	LEIN-I-733-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Antibodies Flow Cytometry Western Blot ELISA Primary Antibodies Immunostaining
Type	Antibody Primary
Applications	WB, FC, ELISA
Specific against	Human (Homo sapiens)
Host	Sheep
Citations	1. Simmons, DL. et al. (1992) Nature 360: 481 2. Mason, DY. et al. (1994) J Clin Pathol 47: 143
NCBI	3385
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	ELISA Indirect, FC, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	ICAM-3
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50) is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low.1 ICAM-3 is closely related to ICAM-1. 1 ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.2
Background	Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50) is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins that bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signaling molecule. ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low.¹ ICAM-3 is closely related to ICAM-1. ¹ ICAM-3 is essentially restricted to the haemopoietic system and is reciprocal in its expression to ICAM-1, in that it is present on resting cells and its level falls as a result of cell activation.²
Other Applications Reported In Literature	CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	ICAM-3
Manufacturer - Specificity	Sheep Anti-Human Intracellular Adhesion Molecule 3 (ICAM-3) recognizes Human ICAM-3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2830854
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	CHO Cell-Derived Recombinant Human ICAM-3 Extracellular Domain
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Intracellular Adhesion Molecule 3, CD50, CDW50, ICAM-R, ICAM-3, Human ICAM-3, Anti-Human ICAM-3, CD50 antigen, CD50, CDW50CDw50, ICAM3, ICAM-3, ICAM-Rintercellular adhesion molecule-3, intercellular adhesion molecule 3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-733-100ug-safety-datasheet.pdf