Anti-Mouse L-Selectin (Clone 95205) - 25 µg

Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing L-Selectin. A suggested method would be to stain cells expressing L-Selectin with 0.25 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:R485.
Western Blotting: To detect Mouse L-Selectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse L-Selectin is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

L-Selectin

Next Day Ambient

L-selectin, also known as CD62L, is a cell adhesion molecule consisting of a large, highly glycosylated, extracellular domain1 found on leukocytes. It belongs to the selectin family of proteins, which recognise sialylated carbohydrate groups. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via the high endothelial venules. L-selectin is an effective marker for separating lymphoid progenitors from myeloid progenitors and hematopoietic stem cells in bone marrow.2

L-Selectin

AB_2831148

< 0.1 EU/µg as determined by the LAL method

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.