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Anti-Mouse Ly-6G [Clone 1A8] — Purified - 50 µg

Anti-Mouse Ly-6G [Clone 1A8] — Purified - 50 µg

Item no.	LEIN-L279-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Quantity options	0.5 mg 50 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC
Clone	1A8
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Citations	1. Fleming TJ, at al. (1993) J Immunol. 151(5):2399-408 2. Tsetlin VI. (2015) Trends Pharmacol Sci. 36(2):109-23 3. Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70 4. Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594 5. Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328. 6. Stroncek DF. (2007) Curr Opin Hematol. 14(6):688-93 7. Wang JX, et al. (2012) Blood. 120(7):1489-1498
NCBI	546644
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	FC, IHC FF, IHC FFPE, PhenoCycler®
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Ly-6G
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This purified antibody is stable when stored at 2-8°C. Do not freeze.
Product Description	Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1, 4, 5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.
Background	Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein¹. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds². Ly6G is expressed by murine neutrophils regardless of location and activation^{1, 4, 5}. Eosinophils may also express low levels of Ly6G⁵. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia⁶. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism⁷.
PubMed	Ly-6G
Manufacturer - Research Area	Immunology, Inhibitory Molecules, Innate Immunity
Manufacturer - Specificity	Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
RRID	AB_2831190
Concentration	0.5 mg/ml
Formulation	This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	Ly6G is expressed by neutrophils.
Immunogen	Ly-6G-transfected EL4J Cell Line
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Ly-6G, Lymphocyte Antigen 6 Complex, 1A8, Mouse

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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LEIN-L279-50ug-safety-datasheet.pdf