Comparison

Anti-Human MMP-13 (Clone 87512) - 25 µg

Item no. LEIN-M1278-25ug
Manufacturer Leinco Technologies
Amount 25 ug
Quantity options 100 ug 25 ug 500 ug
Category
Type Antibody Monoclonal
Applications WB, IP, IHC, ICC
Clone 87512
Specific against Human (Homo sapiens)
Host Mouse
Citations 1. Knäuper, V. et al. (1996) J Biol. Chem. 271: 17124
2. Krane, SM. et al. (2004) Proc Natl Acad Sci U S A. 101(49):17192-7.
3. Woessner, JF. et al. (2000) J Biol. Chem. 275: 4183
4. Kennedy, AM. et al. (2005) J Clin. Invest. 115: 2832
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping Condition Room temperature
Available
Manufacturer - Applications
ICC, IHC FFPE, IP, WB
Manufacturer - Category
Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets
MMP-13
Country of Origin
USA
Shipping Temperature
Next Day Ambient
Storage Conditions
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description
Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4
Background
Matrix metallopeptidase 13 (collagenase 3), also known as MMP13, is a glycosylated protein1 and a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, which is expressed in the skeleton during embryonic development2 and are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-13 is tightly bound to tissues and utilizes heparan sulfate proteoglycans as extracellular docking molecules. It is produced by many different cell types as an inactive proform that requires proteolytic activation to gain activity.3 Mutations causing lack of MMP-13 expression have been shown to be associated with skeletal disorders characterized by defective growth and modeling of the spine and long bones (spondyloepimetaphyseal dysplasias).4
Other Applications Reported In Literature
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunoprecipitation: This antibody coupled to sepharose beads has been used to immunoprecipitate and immunopurify rhMMP-13 from conditioned media.
Immunocytochemistry: Suitable for use at concentration of 8-25 µg/mL.
PubMed
MMP-13
Manufacturer - Specificity
Mouse Anti-Human Matrix Metalloproteinase 13 (MMP-13) (Clone 87512) recognizes an epitope on Human MMP-13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -10 and -12.
RRID
AB_2831272
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Immunogen
CHO Cell-Derived Recombinant Human MMP-13 (Accession # P45452)
Additional Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords
Monoclonal Antibody, MMP-13, Collagenase 3, CLG3, CL3, 87512, MMP-13, Human, Anti-Human MMP-13, CLG 3, CLG3EC 3.4.24, collagenase 3, EC 3.4.24.-, EC 3.4.24.22, EC 3.4.24.24, EC 3.4.24.35, EC 3.4.24.65, EC 3.4.24.7, MANDP1, matrix metallopeptidase 13 (collagenase 3), matrix metalloproteinase 13 (collagenase 3), Matrix metalloproteinase-13, MMP13, MMP-13

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 25 ug
Available: In stock
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