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Anti-Human Pro/Active MMP-8 - 500 µg

Anti-Human Pro/Active MMP-8 - 500 µg

Item no.	LEIN-M1290-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Immunoprecipitation Western Blot ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Applications	WB, IP, ELISA
Clone	100619
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Klebe, RJ. et al. (2001) Matrix Biol. 20: 577 2. Yager, DR. et al. (1999) J Surg Res. 81: 189 3. Uusitupa, M. et al. (2000) J Periodontal Res. 35: 259
NCBI	4317
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	ELISA Cap, IP, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	MMP-8
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP8 is stored in secondary granules within neutrophils and is activated by autolytic cleavage. MMP8 is a neutral metalloproteinase of the fibrillar collagenase family that also includes MMP-1 and MMP-13. In contrast to the other collagenases, MMP-8 has a very limited tissue distribution, thought to be restricted to neutrophils and chondrocytes.1 Its function is degradation of type I, II and III collagens. MMP-8 is the predominant collagenase present in normal healing wounds and suggests that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers.2 MMP-8 might also be useful in monitoring periodontal disease in diabetes.3
Background	Matrix metallopeptidase 8 (neutrophil collagenase), also known as MMP8, is a part of the MMP family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP8 is stored in secondary granules within neutrophils and is activated by autolytic cleavage. MMP8 is a neutral metalloproteinase of the fibrillar collagenase family that also includes MMP-1 and MMP-13. In contrast to the other collagenases, MMP-8 has a very limited tissue distribution, thought to be restricted to neutrophils and chondrocytes.¹ Its function is degradation of type I, II and III collagens. MMP-8 is the predominant collagenase present in normal healing wounds and suggests that overexpression and activation of this collagenase may be involved in the pathogenesis of nonhealing chronic ulcers.² MMP-8 might also be useful in monitoring periodontal disease in diabetes.³
Other Applications Reported In Literature	Immunoprecipitation: This antibody can be coupled to sepharose beads and used to immunoprecipitate human MMP-8 from conditioned cell culture medium at a concentration of 25 µg/mL.
PubMed	MMP-8
Manufacturer - Specificity	Mouse Anti-Human Matrix Metalloproteinase 8 (MMP-8) (Clone 100619) recognizes an epitope on Human MMP-8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2831285
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human MMP-8 (Accession # AAZ38714)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, MMP-8, CLG1, HNC, PMNL-CL, 100619, MMP-8, Human, Anti-Human Pro/Active MMP-8, CLG1HNC, Collagenase 2, EC 3.4.24, EC 3.4.24.34, matrix metallopeptidase 8 (neutrophil collagenase), matrix metalloproteinase 8 (neutrophil collagenase), Matrix metalloproteinase-8, MMP8, MMP-8, neutrophil collagenase, PMNL collagenase, PMNL-CL

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-M1290-500ug-safety-datasheet.pdf