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Anti-Human CD20 - 25 µg

Anti-Human CD20 - 25 µg

Item no.	LEIN-M1343-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC
Clone	396444
Specific against	Human (Homo sapiens)
Host	Mouse
NCBI	931
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, FC, IHC
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines\|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers
Manufacturer - Targets	CD20
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.
Background	CD20 is a 33-37 kD transmembrane-spanning phosphoprotein that facilitates optimal B-cell immune response against T-independent antigens. It can exist in a complex with MHC class I, MHC class II, CD53, CD81, and CD82. However, CD20 can also form homo-oligomers. It has been suggested that homo-oligomerization of CD20 forms calcium ion channels in the plasma membrane of B cells. CD20 can be useful in diagnosing B-cell lymphomas and leukemias, and is the target of mAbs in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases. Interestingly, studies show a link between the immune system's B cells and diabetes mellitus in which anti-CD20 antibodies rendered the T cell antibodies dysfunctional and, hence, unable to cause insulin desensitivity by a B cell antibody-modulated autoimmune response.
Function	B cell activation
Other Applications Reported In Literature	Immunohistochemistry:5-25 µg/mL CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	CD20
Ligand/Receptor	Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Manufacturer - Specificity	Clone 396444 recognizes an epitope on human CD20.
RRID	AB_2831317
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Antigen Distribution	CD20 is present on human pre B lymphocytes and on B lymphocytes, except on plasma cells, and is expressed in tandem with surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset.
Immunogen	Purified Recombinant Human MS4A (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, MS4A, CD20, Clone 396444, Human MS4A, Anti-Human MS4A, Human CD20, Anti-Human CD20

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-M1343-25ug-safety-datasheet.pdf