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Anti-Human MCP-3 (Clone 36320) - 500 µg

Anti-Human MCP-3 (Clone 36320) - 500 µg

Item no.	LEIN-M153-500ug
Manufacturer	Leinco Technologies
Amount	500 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Flow Cytometry Western Blot ELISA Primary Antibodies Immunostaining
Type	Antibody Monoclonal
Applications	WB, FC, ELISA
Clone	36320
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Van Damme, J. et al. (1993) Biochem. Biophys. Res. Commun. 191: 535 2. Parmentier, M. et al. (1999) Blood 94: 1899 3. Brosnan, CF. et al. (1998) J Neuroimmunol. 86: 20 4. Reddigari, SR. et al. (1995) Exp. Dermatol. 4: 260 5. Matikainen, S. et al. (2001) Cytokine Growth Factor Rev. 12: 171
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	CyTOF®, ELISA Cap, FC, N, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	MCP-3
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.1 MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes. MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.2 MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.3, 4, 5
Background	Monocyte chemotactic protein-3 (MCP3) is a non-glycosylated polypeptide belonging to the the CC-Chemokines family that attracts monocytes and eosinophils, augments monocyte anti-tumor activity, and induces the release of gelatinase B. It also regulates protease secretion by macrophages. MCP3 can bind heparin.¹ MCP-3 is one of the most pluripotent chemokines since it activates all types of leukocytes. MCP-3 may affect HIV-1 infection via inhibiting the binding of HIV envelope to CCR5.² MCP-3 has also been implicated in Multiple Sclerosis, allergic reactions and viral infections.^{3, 4, 5}
Other Applications Reported In Literature	Neutralization: This antibody is useful for neutralization of Human MCP-3 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human MCP-3 (at 1.0 ng/ml) is 1.5 - 4.5 µg/ml. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
PubMed	MCP-3
Manufacturer - Specificity	Mouse Anti-Human Monocyte Chemotactic Protein-3 (MCP-3) (Clone 36320) recognizes an epitope on Human MCP-3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2831357
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	E. coli-Derived Recombinant Human CCL7 (Accession # Q7Z7Q8)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, MCP-3, CCL7, SCYA6, SCYA7, Small Inducible Cytokine A7, CCL7, NC28, Chemokine (C-C Motif) Ligand 7, FIC, MARC, MGC138463, MGC138465, 36320, MCP-3, Human, Anti-Human MCP-3, C-C motif chemokine 7, CCL7, chemokine (C-C motif) ligand 7, FIC, MARC, MCP-3, MCP3Monocyte chemotactic protein 3, MCP-3Small-inducible cytokine A7, MGC138465, Monocyte chemoattractant protein 3, NC28SCYA6, SCYA7MGC138463, small inducible cytokine A7 (monocyte chemotactic protein 3)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-M153-500ug-safety-datasheet.pdf