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Anti-Human PDGF-AA - 25 µg

Anti-Human PDGF-AA - 25 µg

Item no.	LEIN-P121-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Canalis, E. et al. (1994) Endocrin. 134: 2541 2. Deuel, TF. et al. (1992) Biochem Biophys Res Commun. 186: 1510 3. Post, M. et al. (1995) Endocrin. 121: 25529 4. Sueishi, K. et al. (2005) Cancer Research 65: 7241
NCBI	5154
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	IHC FFPE, N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	PDGF-AA
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Platelet-derived growth factor (PDGF), an agent with important mitogenic effects for bone cells, exists in three isoforms, PDGF-AA, - BB, and –AB.1 The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells.2 PDGF AA is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle, and connective tissue. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.3 PDGF AA is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression. It regulates the frequency and level of VEGF expression during the transition from a precancerous lesion to advanced cancer.4
Background	Platelet-derived growth factor (PDGF), an agent with important mitogenic effects for bone cells, exists in three isoforms, PDGF-AA, - BB, and –AB.¹ The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells.² PDGF AA is a potent mitogen for a wide range of cell types including fibroblast, smooth muscle, and connective tissue. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.³ PDGF AA is also an essential autocrine regulator for vascular endothelial growth factor (VEGF) expression. It regulates the frequency and level of VEGF expression during the transition from a precancerous lesion to advanced cancer.⁴
Other Applications Reported In Literature	Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL. Neutralization:Measured by its ability to neutralize PDGF‑AA-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The Neutralization Dose (ND₅₀) is typically 0.1-0.3 µg/mL in the presence of 25 ng/mL Recombinant Human PDGF‑AA.
PubMed	PDGF-AA
Manufacturer - Specificity	Rabbit Anti-Human Platelet Derived Growth Factor-AA (PDGF-AA ) recognizes Human PDGF-AA . This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831520
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human PDGF-AA (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, PDGF-AA, Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), PDGF-AA, Human PDGF-AA, Anti-Human PDGF-AA, PDGFAA, PDGF-AA

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-P121-25ug-safety-datasheet.pdf