Anti-PCNA - DyLight® 680

Quality Tested by Leinco FC The suggested concentration for this anti-PCNA (Clone PC-10) antibody for staining cells in flow cytometry is ≤ 5.0 μl per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

PCNA

Next Day 2-8°C

PCNA antibody, clone PC10, recognizes proliferating cell nuclear antigen (PCNA), also known as cyclin, a 36 kDa nuclear protein belonging to the DNA sliding clamp family. PCNA is a ring-shaped homotrimer that encircles and slides along DNA1, 2. PCNA is an auxiliary protein of DNA polymerase δ and ε and tethers the polymerase catalytic unit to the DNA template for efficient DNA synthesis3-6. PCNA also contributes to chromatin remodeling, DNA repair, sister chromatid cohesion, and cell cycle control7. Expression levels of PCNA are associated with proliferation and transformation, and PCNA is commonly used as a marker for proliferating cells8, 9. Antibodies to PCNA are detected in sera from patients with systemic lupus erythematosus (SLE)10. In addition, PCNA is a reliable diagnostic and prognostic biomarker for cancer, as tumor cells express high levels of PCNA11.

Increases DNA polymerase δ processibility during elongation of the leading strand.

PCNA

Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cel

AB_2831622

This DyLight® 680 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Rat PCNA made in the protein A vector pR1T2T