Comparison

Anti-PolyHistidine [AD1.1.10] – Dylight® 650 - 50 µg

Item no. LEIN-P399-50ug
Manufacturer Leinco Technologies
Amount 50 ug
Quantity options 250 ug 50 ug
Category
Type Antibody Monoclonal
Applications WB, FC, IF, IP, ELISA
Clone AD1.1.10
Specific against other
Formula This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping Condition Room temperature
Available
Manufacturer - Category
Primary Monoclonal Antibodies>Epitope Tag
Manufacturer - Targets
PolyHistidine
Shipping Temperature
Next Day Ambient
Storage Conditions
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description
AD1.1.10 recognizes proteins and peptides containing the polyhistidine tag (His-tag). The most common His-tag is the H-H-H-H-H-H (6x-His) motif, but His-tags can comprise up to 10 His residues. His-tags can be inserted into a target gene either by cloning the encoding DNA in frame into a His-tag containing vector or by using extended polymerase chain reaction primers that incorporate the motif. His-tags can be inserted at either the N or C terminal of target proteins. The most suitable location depends on the target protein’s characteristics as well as the downstream experimental methods. A variety of purification and detection methods make use of His-tags. Applications include flow cytometry, ELISA, Western blot, dot blot, peptide array, immunohistochemistry, immunofluorescence, immunoprecipitation, immunocytochemistry, histidine tag (His-tag) insertion for epitope mapping (HisMAP), and chromatin immunoprecipitation (ChIP). His-tags are less likely than other tags to affect functionality of the fusion protein due to their relatively small size. However, His residues exist in mammalian and insect systems, and therefore there may be some background binding, depending on the experimental conditions and/or the system used.
Manufacturer - Research Area
Epitope Tag, Protein Purification
Concentration
0.2 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution
The 6His motif is present in histidine tags.
Protein
PolyHistidine
Keywords
Monoclonal Antibody, PolyHistidine, AD1.1.10, Anti-PolyHistidine Antibody, Clone AD1.1.10

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
available

Delivery expected until 11/6/2025 

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