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Anti-Human TGF-α - 25 µg

Anti-Human TGF-α - 25 µg

Item no.	LEIN-T145-25ug
Manufacturer	Leinco Technologies
Amount	25 ug
Quantity options	100 ug 25 ug
Category	Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies Immunostaining
Type	Antibody Primary
Applications	WB, IHC, ELISA
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Taylor, HS. et al. (2009) Semin Reprod Med.27(1):62-79 2. Lamaroon, A. et al. (2002) J Dental Research 75:1534 3. Loughlin, S. et al. (2002) Proc Natl Acad Sci USA 97:14686 4. Fuchs, E. et al. (1991) Genes & Dev. 5:714
NCBI	7039
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	ELISA Cap, IHC FFPE, N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	TGF-alpha
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Transforming growth factor alpha (TGF-α) is thought to be the major autocrine factor controlling growth in epidermal cells.1 It plays an important role in cell proliferation during embryogenesis.2 It also stimulates neural cell proliferation in the adult injured brain.3 TGF-alpha is an oncogenic growth factor which drives estrogen independent breast tumor proliferation via an autocrine loop.4 TGF-alpha which normally suppresses tumours can actually be used by cancer cells to enhance their ability to spread. It is present in high levels in around half of all breast tumours, and these tumours tend to be more aggressive and more likely to spread to the lungs. It has been shown that TGF-alpha can prompt breast cancer cells to make another cytokine called angiopoietin-like 4 (ANGPTL4), which helps the cells to spread through the bloodstream.5
Background	Transforming growth factor alpha (TGF-α) is thought to be the major autocrine factor controlling growth in epidermal cells.¹ It plays an important role in cell proliferation during embryogenesis.² It also stimulates neural cell proliferation in the adult injured brain.³ TGF-alpha is an oncogenic growth factor which drives estrogen independent breast tumor proliferation via an autocrine loop.⁴ TGF-alpha which normally suppresses tumours can actually be used by cancer cells to enhance their ability to spread. It is present in high levels in around half of all breast tumours, and these tumours tend to be more aggressive and more likely to spread to the lungs. It has been shown that TGF-alpha can prompt breast cancer cells to make another cytokine called angiopoietin-like 4 (ANGPTL4), which helps the cells to spread through the bloodstream.⁵
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose) is typically 0.15-0.75 μg/mL in the presence of 3 ng/mL Recombinant Human TGF‑ alpha.
PubMed	TGF-alpha
Manufacturer - Specificity	Rabbit Anti-Human Transforming Growth Factor-Alpha (TGF-α) recognizes Human TGF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831827
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human TGF-α (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Transforming Growth Factor-Alpha, Sacroma Growth Factor, TGF-Type I, ETGF, MDGF-2 (Milk-Derived Growth Factor-2), TCGF Transformed Cell Growth Factor, TGF-α, Human TGF-α, Anti-Human TGF-α, protransforming growth factor alpha, TFGA, TGFA, TGFalpha, TGF-alpha, transforming growth factor, alpha, transforming growth factor-alpha

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T145-25ug-safety-datasheet.pdf