Anti-Human TNF-β - 100 µg

Item no.	LEIN-T147-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Antibodies By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB
Specific against	Human (Homo sapiens)
Host	Caprine
Citations	1. Adolf, GR. et al. (1990) Infec Immun. 58:3996 2. Pandey, JP. et al. (2002) Autoimmunity. 35:377
NCBI	4049
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	N, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	TNF-beta
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Tumor necrosis factor-beta (TNF beta) also known as lymphotoxin alpha (LT alpha), is a multifaceted pro-inflammatory cytokine which may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.1 TNF beta has been implicated in the pathogenesis of several autoimmune diseases and it plays a significant role in the etiopathogenesis of multiple sclerosis (MS).2
Background	Tumor necrosis factor-beta (TNF beta) also known as lymphotoxin alpha (LT alpha), is a multifaceted pro-inflammatory cytokine which may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites.¹ TNF beta has been implicated in the pathogenesis of several autoimmune diseases and it plays a significant role in the etiopathogenesis of multiple sclerosis (MS).²
Other Applications Reported In Literature	Neutralization:Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221]. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and 1 µg/mL actinomycin D.
PubMed	TNF-beta
Manufacturer - Specificity	Rabbit Anti-Human Tumor Necrosis Factor-Beta (TNF-β) recognizes Human TNF-β. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831829
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human TNF-β (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Tumor Necrosis Factor-Beta, Lymphotoxin-Alpha (LT Alpha), Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha 3, TNF-β, Human TNF-β, Anti-Human TNF-β, LT, LTA, LT-alpha, lymphotoxin alpha TNF superfamily, member 1, Lymphotoxin alpha, Lymphotoxinalpha, Lymphotoxin-alpha, tnfb, TNF-beta, TNFBlymphotoxin-alpha, TNFSF1, TNFSF1B, TNFSF1TNF-beta, tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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LEIN-T147-100ug-safety-datasheet.pdf