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Anti-Human Tuberous Sclerosis Complex 1 (CT) (TSC1) - 0.1 mg

Anti-Human Tuberous Sclerosis Complex 1 (CT) (TSC1) - 0.1 mg

Item no.	LEIN-T291-0.1mg
Manufacturer	Leinco Technologies
Amount	0.1 mg
Quantity options	0.1 mg 20 ug
Category	Oncology Antibodies By Cell Type Carcinoma Hematology Lymphoma Myeloma Adenocarcinoma Western Blot Primary Antibodies Astrocytoma
Type	Antibody Primary
Applications	WB
Specific against	Human (Homo sapiens)
Host	Rabbit
Citations	1. Shamji, AF. et al. (2003) Mol. Cell 12:271 2. Inoki, K. et al. (2005) Mol. Biol. Rev. 69:79 3. Tabancay Jr., AP. et al. (2003) J. Biol. Chem. 278:39921 4. Inoki, K. et al. (2003) Genes Dev. 17:1829
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Category	Primary Monoclonal Antibodies>Apoptosis & Cell Biology
Manufacturer - Targets	TSC1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Product Description	Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1, 2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high.3 Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, 4 suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth.4 A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
Background	Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1, 2). TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high.³ Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, ⁴ suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth.⁴ A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
PubMed	TSC1
Manufacturer - Specificity	Rabbit Anti-Human Tuberous Sclerosis Complex 1 (TSC1) recognizes an epitope near the C-terminus of Human and Mouse TSC1. This polyclonal antibody was purified using affinity chromatography.
RRID	AB_2831888
Concentration	0.5 mg/ml
Formulation	This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	TSC1 Antibody, LAM, TSC, KIAA0243, Hamartin, Tuberous sclerosis 1 protein

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LEIN-T291-0.1mg-safety-datasheet.pdf