Anti-Mouse TIRAP (CT)

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rabbit

Immunogen

PN:T363

Product Concentration

0.5 mg/ml

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Handling

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2831910

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rabbit Anti-Mouse TIRAP recognizes an epitope near the C-terminus of human, mouse and rat TIRAP. This polyclonal antibody was purified using affinity chromatography.

Background

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4, 5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-?B and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6, 7)

Antigen Details

Protein

TIRAP

PubMed

TIRAP

References & Citations

1. Fenton, MJ. et al. (2003) Mol. Interv. 3:466-77.
2. Akira, S. et al. (2003) Annu.Rev. Immunol. 21:335-76.
3. Janeway Jr., CA. and Medzhitov, R. (2002) Annu. Rev. Immunol. 20:197-216.
4. Bowie, AG. et al. (2003) Trends in Imm. 24:286-9.
5. McGettrick, AF. and O’Neill, LAJ. (2004) MolImm. 41:577-82.
6. Hemmi, H. et al. (2002) Nature 420:324-9.
7. Flavell, RA. et al. (2002) Nature 420:329-333.

Technical Protocols