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Anti-Human Thrombopoietin

Anti-Human Thrombopoietin

Item no.	LEIN-T432-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug 500 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	ELISA
Clone	34817
Specific against	Human (Homo sapiens)
Host	Mouse
Citations	1. Vainchenker, W. et al. (1998) Eur Cytokine Newt. 9: 221 2. Skoda, RC. et al. (1998) Blood 92: 4023
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:T195 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:T155.
Manufacturer - Category	Primary Monoclonal Antibodies>Growth Factors and Cytokines
Manufacturer - Targets	Thrombopoietin
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Product Description	Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.1 TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis2 and platelet production.1
Background	Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine.¹ TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis² and platelet production.¹
Other Applications Reported In Literature	Neutralization: This antibody is useful for neutralization of Human TPO bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human TPO (at 10 ng/ml) is 5-10 µg/ml.
PubMed	Thrombopoietin
Manufacturer - Specificity	Mouse Anti-Human Thrombopoietin (TPO) (Clone 34817) recognizes an epitope on Human TPO. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2831932
Formulation	This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	Purified Recombinant Human TPO (Accession # P40225)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T432-100ug-safety-datasheet.pdf