Anti-Rat TNF-α (Clone 45418) - 500 µg

ELISA Cap, N, WB

TNF-α

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TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Neutralization: This antibody is useful for neutralization of Rat TNF-α bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Rat TNF-α (at 0.025 ng/ml) is 10 - 40 µg/ml.

Clone 45418 recognizes an epitope on rat TNF-α. This antibody shows 100% cross-reactivity with rmTNF-α and no cross-reactivity with rfeTNF-α.

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

< 0.1 EU/µg as determined by the LAL method

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.