« Back
Home /
Anti-Rat TIMP-1 - 100 µg

Anti-Rat TIMP-1 - 100 µg

Item no.	LEIN-T467-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 25 ug
Category	Antibodies Immunohistochemistry Immunoprecipitation Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, IP, IHC
Specific against	Rat (Rattus norvegicus)
Host	Caprine
Citations	1. Denhardt, DT.et al. (2005) Breast Cancer Res Treat. 94: 185 2. Khokha, R. et al. (2005) Hepatology 41: 857 3. Chen, X. et al. (2008) Nephrology Dialysis Transportation 23: 1861
NCBI	116510
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	IHC FFPE, IP, WB
Manufacturer - Category	Polyclonal Antibodies>Growth Factors & Cytokines
Manufacturer - Targets	TIMP-1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Product Description	Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.1 TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.2 Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.3
Background	Tissue Inhibitor of Metalloproteinases 1, also known as TIMP1 is a widely expressed, secreted protein that functions primarily to inhibit members of a large family of metalloproteinases (MPs). Because of the ability of TIMP-1 to inhibit MPs, it functions in many of the same pathophysiological processes as these enzymes, e.g. wound healing, ovulation, angiogenesis, and cancer cell metastasis. TIMP-1 can also stimulate proliferation and cellular anabolic processes.¹ TIMP-1 is a novel negative regulator of HGF activity during liver regeneration.² Overexpression of TIMP-1 could promote renal interstitial fibrosis through the inflammatory pathway, which might be partly induced by upregulating ICAM-1.³
Other Applications Reported In Literature	IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/mL. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Immunoprecipitation: The antibody has been used at 25 μg/mL to immunoprecipitate rrTIMP-1 from conditioned media of transfected NS0 cells.
PubMed	TIMP-1
Manufacturer - Specificity	Goat Anti-Rat Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) recognizes Rat TIMP-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
RRID	AB_2831967
Formulation	This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Endotoxin Level	< 0.1 EU/µg as determined by the LAL method
Immunogen	NS0-Derived Recombinant Rat TIMP-1
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, Tissue Inhibitor of Metalloproteinases 1, EPO, CLGI, EPA, FLJ90373, HCI, TIMP, TIMP-1, Rat TIMP-1, Anti-Rat TIMP-1, CLGI, Collagenase inhibitor, collagenase inhibitor, EPATIMP-1, EPO, erythroid potentiating activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, metalloproteinase inhibitor 1, TIMP metallopeptidase inhibitor 1, TIMP1, TIMP-1, TIMPtissue inhibitor of metalloproteinase 1 erythroid potentiating activity, Tissue inhibitor of metalloproteinases 1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

LEIN-T467-100ug-safety-datasheet.pdf