Anti-Human Tie-1

Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing Tie 1. A suggested method would be to stain cells expressing Tie 1 with 0.50 µg per 1 - 2.5 x 10⁶ cells in a total staining volume of ≤200 µl followed by PN:G641.
Western Blotting: To detect Human Tie 1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rhTie 1 is approximately 5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Tie-1

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Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie-1) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.1 They are expressed almost exclusively in endothelial cells.2Tie-1 also have a role in the regulatory pathways of early hematopoiesis and angiogenesis.3 It is upregulated in diseases such as atherosclerosis and rheumatoid arthritis.4

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
CyTOF-ready:Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Goat Anti-Human Tie 1 recognizes Human Tie 1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.