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Anti-Mouse TER-119 (Erythroid Cells) - APC

Anti-Mouse TER-119 (Erythroid Cells) - APC

Item no.	LEIN-T654-100ug
Manufacturer	Leinco Technologies
Amount	100 ug
Quantity options	100 ug 200 ug 25 ug 500 ug 50 ug
Category	Antibodies Flow Cytometry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC
Clone	TER-119
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
NCBI	104231
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Category	Recombinant Antibodies\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	TER-119
Country of Origin	USA
Shipping Temperature	2 - 8°C Wet Ice
Storage Conditions	This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	TER-119 recognizes a 52 kDa cell surface protein that strongly associates with glycophorin A on the erythrocyte membrane 1 . The antigen is not present on a number of tested cell lines, including T cell, B cell, macrophage, mast cell, fibroblast, epithelial, and murine erythroleukemia cultured lines. TER-119 is a mouse erythroid lineage-specific rat monoclonal antibody that reacts with 100% of mature erythrocytes and 80-90% of day 14 fetal liver cells, after which the percentage of TER-119+ fetal cells declines to 40-50% 2. Additionally, TER-119 stains 20-25% of adult mouse bone marrow cells and 2-3% of spleen cells, but not thymocytes or lymph node cells. TER-119 was produced in Wister rats immunized with BALB/c fetal liver cells 1 . Spleen cells were fused with X63.Ag8.653 myeloma cells to produce a hybridoma clone reactive against fetal liver cells. TER-119 recognizes a 7, 9 di-O-acetyl sialic acid glyco-epitope 3 . Additionally, the epitope is sensitive to hemagglutinin esterase derived from bovine coronavirus (Mebus strain) but not influenza C.
Background	TER-119 is also known as Lymphocyte antigen 76 (Ly76) and is known to associate with the 52 kDa glycophorin A protein.
PubMed	TER-119
Excitation Laser	Red Laser (650 nm)
Manufacturer - Research Area	Immunology
Manufacturer - Specificity	Rat Anti-Mouse TER-119 (Clone TER-119) recognizes an epitope on Mouse TER-119. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2832096
Concentration	0.2 mg/ml
Formulation	This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	TER-119 is highly specific for erythroid cells, from early proerythroblasts to mature erythrocytes. TER-119 antigen is expressed on normal erythroid cells, but not erythroleukemia cells, even when induced with dimethylsulfoxide. TER-119 is not thought to interact with pluripotent hematopoietic stem cells in adult bone marrow.
Immunogen	Purified Recombinant Mouse TER-119 (> 98%)
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T654-100ug-safety-datasheet.pdf