Anti-Mouse TCR β chain - FITC - 50 µg

Item no.	LEIN-T663-50ug
Manufacturer	Leinco Technologies
Amount	50 ug
Quantity options	500 ug 50 ug
Category	Antibodies Flow Cytometry Immunohistochemistry Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC
Clone	H57-597
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
Citations	1. Krijgsman D, et al. (2018) Front Immunol. 9:367 2. Clevers H, et al. (1988) Annu Rev Immunol. 6:629-62 3. Kuhns MS, Badgandi HB. (2012) Immunol Rev. 250(1):120-43 4. Wucherpfennig KW, et al. (2010) Cold Spring Harb Perspect Biol. 2(4):a005140 5. Samelson LE. (2002) Annu Rev Immunol. 20:371-94 6. Borroto A, et al. (2014) Immunol Lett. 161(1):113-7
NCBI	21577
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	FC, IHC FF
Manufacturer - Category	Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	TCR beta
Country of Origin	USA
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Product Description	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells1. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond2. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling3, 4. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival5, 6.
Background	TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells¹. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond². TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling^{3, 4}. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival^{5, 6}.
Function	Antigen recognition, T cell activation
Other Applications Reported In Literature	IHC FF
PubMed	TCR beta
Excitation Laser	Blue Laser (490 nm)
Ligand/Receptor	Peptide bound-MHC class I and II
Manufacturer - Specificity	Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.
RRID	AB_2832105
Concentration	0.2 mg/ml
Formulation	This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Antigen Distribution	TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.
Immunogen	Affinity purified TCR from mouse DO-11.10 cells
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, TCR beta, TCR-β chain, TCR-β, β-TCR, H57-597, Mouse, Anti-Mouse β (TCR) - FITC, FITC, Flow Cytometry, Immunology

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LEIN-T663-50ug-safety-datasheet.pdf