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Anti-Mouse CD366 (Tim-3) - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 1.0 mg

Anti-Mouse CD366 (Tim-3) - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 1.0 mg

Item no.	LEIN-T720-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies Flow Cytometry Immunohistochemistry Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Applications	FC, IF, IHC, other, IV
Clone	RMT3-23
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	≥98% monomer by analytical SEC, >95% by SDS Page
Citations	1. Monney, L. et al. (2002)Nature 415, 536–541. 2. Gao, X. et al. (2012) PLOS ONE 7, e30676. 3. Anderson, A. C. et al. (2007) Science 318, 1141–1143. 4. Ndhlovu, L. C. et al. (2012) Blood 119, 3734–3743. 5. Phong, B. L. et al. J. (2015) Exp. Med. 212, 2289–2304. 6. Li, X. et al. (2010) Clin. Immunol. 134, 169–177. 7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4, 1093–1101. 8. Zhu C, et al.. Nature Immunology. 2005;6:1245–1252.
NCBI	171285
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Applications	B, FC, IF Staining, IHC, IHC FF, in vivo, PhenoCycler®
Manufacturer - Category	Primary Monoclonal Antibodies>Immune Checkpoints\|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade
Manufacturer - Targets	Tim-3
Shipping Temperature	Next Day 2 - 8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells1, regulatory T cells (Tregs)2, myeloid cells3, NK cells4, and mast cells5. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)1, inflammatory bowel disease (IBD)6, and type I diabetes7. Tim-3 is also proposed to induce immunological tolerance7, promote Th1 apoptosis8, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer9.
Background	Tim-3 antibody, clone RMT3-23, recognizes T cell immunoglobulin and mucin domain-containing protein 3 (TIM3, also known as CD366), a TIM family member of immunoregulatory proteins. Tim-3 is a 60kDa type I transmembrane protein with an extracellular immunoglobulin and mucin-like domain and a cytoplasmic tyrosine phosphorylation motif. Tim-3 is expressed by IFNg-producing Th1 CD4 and Tc1 (cytotoxic) CD8 T cells¹, regulatory T cells (Tregs)², myeloid cells³, NK cells⁴, and mast cells⁵. Tim-3 is an inhibitory molecule that limits Th1-mediated inflammatory diseases, including a model of central nervous system inflammation (experimental autoimmune encephalomyelitis, EAE)¹, inflammatory bowel disease (IBD)⁶, and type I diabetes⁷. Tim-3 is also proposed to induce immunological tolerance⁷, promote Th1 apoptosis⁸, and regulate macrophage activation1. In addition, Tim-3 is upregulated on tumor-infiltrating lymphocytes, and coblockade of Tim-3 and immune checkpoint inhibitors, such as PD-1, is currently being investigated in clinical trials for the treatment of cancer⁹.
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Function	May play a role in the development of immune responses and the development of Th1-mediated responses
Other Applications Reported In Literature	B IHC
PubMed	Tim-3
Ligand/Receptor	Putative ligand on resting CD4+ lymphocytes
Manufacturer - Research Area	Immunology, Inhibitory Molecules
Manufacturer - Specificity	Clone RMT2-23 recognizes an epitope on mouse Tim-3.
RRID	AB_2894397
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Tim-3 is expressed on activated Th1 and Tc1 lymphocytes, CD11b+ macrophages, Tregs, NK cells, and mast cells.
Immunogen	This antibody was produced using recombinant mouse TIM-3
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Monoclonal Antibody, Tim-3, Havcr2, RMT2-23, Mouse, Anti-Mouse Tim-3 - Purified Functional Grade, CD366, FLJ14428, HAVCR2, HAVcr-2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, TIM3 T-cell membrane protein 3, TIM3, TIM-3, TIMD-3, TIMD3KIM-3

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-T720-1.0mg-safety-datasheet.pdf