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Anti-Human XAF-1 (CT) - 0.1 mg

Anti-Human XAF-1 (CT) - 0.1 mg

Item no.	LEIN-X103-0.1mg
Manufacturer	Leinco Technologies
Amount	0.1 mg
Quantity options	0.1 mg 20 ug
Category	Antibodies Immunohistochemistry Western Blot Primary Antibodies
Type	Antibody Primary
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Citations	1. Liston, P. et al. (2001) Nature Cell Biol. 3:128 2. Deveraux, QL. et al. (1997) Nature 388:300 3. Fong, WG. et al. (2000) Genomics 70:113 4. Leaman, DW. et al. (2002) J. Biol. Chem. 277:28504
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Manufacturer - Applications	IHC FFPE, WB
Manufacturer - Category	Primary Monoclonal Antibodies>Apoptosis & Cell Biology
Manufacturer - Targets	XAF-1
Country of Origin	USA
Shipping Temperature	Next Day Ambient
Storage Conditions	This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Product Description	XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus.1, 2 Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death.1 XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines.3 In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines.2, 3 Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.4
Background	XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus.^{1, 2} Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death.¹ XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines.³ In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines.^{2, 3} Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.⁴
PubMed	XAF-1
Manufacturer - Specificity	Rabbit Anti-Human XAF-1 recognizes an epitope near the C-terminus of Human and Mouse XAF-1. This polyclonal antibody was purified using affinity chromatography.
RRID	AB_2832188
Concentration	0.5 mg/ml
Formulation	This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Immunogen	PN:X108
Additional Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Keywords	Polyclonal Antibody, XAF-1, Human, Anti-Human XAF-1 (CT), XAF-1 Antibody, BIRC4BP, XIAPAF1, HSXIAPAF1, BIRC4BP, XIAP-associated factor 1, BIRC4-binding protein

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-X103-0.1mg-safety-datasheet.pdf