Anti-NFL (Neurofilament Light Chain) Antibody

Primary Antibody

Neurofilament Light Chain (NFL)

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

300 µg/mL

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

We produce our Anti-Neurofilament Light Chain (NFL) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is affinity-purified IgY.

AB_2313553

1:1000-1:2000

1:1000-1:2000

Human, Mouse, Rat

12 months from receipt of product

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the NF-L gene product, but are shared between the human (P07196, NCBI) and mouse (P08551, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.