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Human Calmodulin 2/CALM2 antibody

Human Calmodulin 2/CALM2 antibody

Item no.	NKM-ACA0830-50ul
Manufacturer	NKMAX
Amount	50 ul
Quantity options	100 ul 50 ul
Category	Neuroscience Molecular Targets for Neuroscience Antibodies Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, ICC, ELISA
Clone	J4D8
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
Citations	Zhang M., et al. (1998). Biochem Cell Biol. 76(2-3):313-23. James.P., et al. (1995). Trend Biochem Sci. 20: 38-42.
NCBI	NP_001734
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	PHKD,CAMII,PHKD2,Calmodulin 2,CALM 2,Calmodulin2,CAM II,LP7057 protein,PHKD 2,Phosphorylase kinase delta,Calmodulin-2,CALM2
Similar products	Calmodulin, ACA0830-10ug, ACA0830-20ug, ACA0830-50ug, ACA0830-100ug, ACA0830-250ug, ACA0830-500ug, ACA0830-1mg, ACA0830-10, ACA0830-20, ACA0830-50, ACA0830-100, ACA0830-250, ACA0830-500, ACA0830-1
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human Calmodulin (1-149aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-G affinity chromatography
Gene Category	Signal Transduction
Manufacturer - Search Terms	Calmodulin, ACA0830-10ug, ACA0830-20ug, ACA0830-50ug, ACA0830-100ug, ACA0830-250ug, ACA0830-500ug, ACA0830-1mg, ACA0830-10, ACA0830-20, ACA0830-50, ACA0830-100, ACA0830-250, ACA0830-500, ACA0830-1
IF Comment	ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: Ramos cell lysate Lane 7.: PC3 cell lysate
FACS Comment	Flow cytometry analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 ul

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Delivery expected until 12/4/2025

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