Comparison

Human C-Reactive Protein/CRP antibody

Item no. NKM-ACR0631-50ul
Manufacturer NKMAX
Amount 50 ul
Quantity options 100 ul 50 ul
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, ICC, ELISA
Clone 5A9
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2a Kappa
Conjugate/Tag Unconjugated
Citations Alexander J. S et al., (2000) Infect Immun 68(10):5652-5656.
Schillaci G. et al., (2006) Nutr Metab Cardiovasc Dis. 16(7):500-8.
NCBI NP_000558
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias C-reactive protein(pentraxin-related),PTX1,MGC88244,MGC149895,C-reactive protein pentraxin-related,pentraxin 1
Similar products CRP, C-Reactive Protein, ACR0631-10ug, ACR0631-20ug, ACR0631-50ug, ACR0631-100ug, ACR0631-250ug, ACR0631-500ug, ACR0631-1mg, ACR0631-10, ACR0631-20, ACR0631-50, ACR0631-100, ACR0631-250, ACR0631-500, ACR0631-1
Available
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
C-reactive protein (CRP) is an acute-phase protein with a well-known association with infection and other inflammatory conditions. Mild elevations in C-reactive protein concentration predict myocardial infarction, stroke, and vascular death in a variety of clinical settings. Despite the lack of specific evidence that C-reactive protein levels are independently associated with cardiovascular risk in patients with hypertension, the prognostic value of C-reactive protein has proven to be complementary to that of blood pressure values.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human CRP (19-224aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Apoptosis
Manufacturer - Search Terms
C-Reactive Protein, CRP, ACR0631-10ug, ACR0631-20ug, ACR0631-50ug, ACR0631-100ug, ACR0631-250ug, ACR0631-500ug, ACR0631-1mg, ACR0631-10, ACR0631-20, ACR0631-50, ACR0631-100, ACR0631-250, ACR0631-500, ACR0631-1
IF Comment
ICC/IF analysis of CRP in HepG2 cells. The cell was stained with ACR0631 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: NIH-3T3 cell lysate
Lane 2.: HepG2 cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HeLa cell lysate
Lane 5.: SH-SY5Y cell lysate
FACS Comment
Flow cytometry analysis of CRP in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ul
Available: In stock
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