Comparison

Human FABP1/L-FABP antibody

Item no. NKM-AFA0908-100ul
Manufacturer NKMAX
Amount 100 ul
Quantity options 100 ul 50 ul
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IHC, ICC, ELISA
Clone 2G4
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Conjugate/Tag Unconjugated
Citations Atshaves BP, et al: (2004) Mol Cell Biochem. 259(1-2), 115-29.
Nakamura T, et al: (2005) Diabestes Care. 28(11), 2728-32.
Schlabritz-Loutsevitch N, et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. (J Med Primatol. 2019)
NCBI NP_001434
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias Fatty acid binding protein 1,Fatty acid-binding protein,liver,FABPL,L-FABP,FABP1,Fatty acid binding protein 1,fatty acid binding protein 1 liver
Similar products L-FABP, FABP1, AFA0908-10ug, AFA0908-20ug, AFA0908-50ug, AFA0908-100ug, AFA0908-250ug, AFA0908-500ug, AFA0908-1mg, AFA0908-10, AFA0908-20, AFA0908-50, AFA0908-100, AFA0908-250, AFA0908-500, AFA0908-1
Available
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF, IHC, FACS
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
FABP1 (Fatty acid binding protein1) encodes the fatty acid binding protein found in liver. FABP1 is composed of ten antiparallelbeta strands that form a barrel with a bigger binding pocket than the other FABPs allowing it to accommodate two fatty acid. This protein binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm; it may be involved in intracellular lipid transport and metabolism.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human FABP1 (1-127aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-G affinity chromatography
Gene Category
Enzymes & Proteases
Manufacturer - Search Terms
FABP1, L-FABP, AFA0908-10ug, AFA0908-20ug, AFA0908-50ug, AFA0908-100ug, AFA0908-250ug, AFA0908-500ug, AFA0908-1mg, AFA0908-10, AFA0908-20, AFA0908-50, AFA0908-100, AFA0908-250, AFA0908-500, AFA0908-1
IF Comment
ICC/IF analysis of FABP1 in Hep3B cells. The cell was stained with AFA0908 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment
The Recombinant protein (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: FABP1 Recombinant protein
Lane 2.: HepG2 cell lysate
Lane 3.: Liver tissues lysate
FACS Comment
Flow cytometry analysis of FABP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IHC Comment
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human FABP1 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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