Human 14-3-3 epsilon antibody

Antibodies

Monoclonal Antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Neuroscience

1433 epsilon, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide, YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14-3-3 epsilon, Tyrosine 3/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide., ATGA0124-10ug, ATGA0124-20ug, ATGA0124-50ug, ATGA0124-100ug, ATGA0124-250ug, ATGA0124-500ug, ATGA0124-1mg, ATGA0124-10, ATGA0124-20, ATGA0124-50, ATGA0124-100, ATGA0124-250, ATGA0124-500, ATGA0124-1

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HeLa cell lysate
Lane 2.: Jurkat cell lysate
Lane 3.: 293T cell lysate
Lane 4.: A549 cell lysate
Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 14-3-3 Zeta Recombinant Protein
Lane 2.: 14-3-3 Beta Recombinant Protein
Lane 3.: 14-3-3 Epsilon Recombinant Protein
Lane 4.: 14-3-3 Eta Recombinant Protein
Lane 5.: 14-3-3 Gamma Recombinant Protein
Lane 6.: 14-3-3 Sigma Recombinant Protein
Lane 7.: 14-3-3 Tau Recombinant Protein