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Human 14-3-3 theta antibody

Human 14-3-3 theta antibody

Item no.	NKM-ATGA0132-50ul
Manufacturer	NKMAX
Amount	50 ul
Quantity options	100 ul 50 ul
Category	Antibodies Cell Biology Cell Status Apoptosis Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, ICC, ELISA
Clone	AT1A1
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
Citations	Liu YC, et al., (1996) J Biol Chem 271:14591-14595. Xiao B, et al., (1995) Nature 376:188-191.
NCBI	NP_006817
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	YWHAQ,14-3-3 protein T-cell,14-3-3 protein tau,Protein HS1,Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta,Protein theta
Similar products	1433 theta, ATGA0132-10ug, ATGA0132-20ug, ATGA0132-50ug, ATGA0132-100ug, ATGA0132-250ug, ATGA0132-500ug, ATGA0132-1mg, ATGA0132-10, ATGA0132-20, ATGA0132-50, ATGA0132-100, ATGA0132-250, ATGA0132-500, ATGA0132-1
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau andtau that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human 14-3-3 tau (1-245aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-G affinity chromatography
Gene Category	Signal Transduction
Manufacturer - Search Terms	1433 theta, ATGA0132-10ug, ATGA0132-20ug, ATGA0132-50ug, ATGA0132-100ug, ATGA0132-250ug, ATGA0132-500ug, ATGA0132-1mg, ATGA0132-10, ATGA0132-20, ATGA0132-50, ATGA0132-100, ATGA0132-250, ATGA0132-500, ATGA0132-1
IF Comment	ICC/IF analysis of 14-3-3 tau in HeLa cells. The cell was stained with ATGA0132 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysate of Jurkat (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human YWHAZ Lane 2. : Recombinant Human YWHAB Lane 3. : Recombinant Human YWHAE Lane 4. : Recombinant Human YWHAH Lane 5. : Recombinant Human YWHAG Lane 6. : Recombinant Human SFN Lane 7. : Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : MDA-MB-21 cell lysate Lane 2. : HeLa cell lysate Lane 3. : A431 cell lysate Lane 4. : NIH3T3 cell lysate Lane 5. : 293T cell lysate Lane 6. : HepG2 cell lysate

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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