Human ERK1/MAPK3 antibody

Antibodies

Monoclonal Antibody

Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Cancer

PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, p44 MAPK, p44 ERK1, Mitogen-activated protein kinase 3, Mitogen activated protein kinase 3, Microtubule-associated protein 2 kinase, Microtubule associated protein 2 kinase, MAPK-3, MAPK3, MAPK 3, MAP kinase3, MAP kinase isoform p44, MAP kinase 3, Insulin-stimulated MAP2 kinase, Insulin stimulated MAP2 kinase, HuMKER1A, HS44KDAP, Extracellular signal-regulated kinase 1, Extracellular signal regulated kinase 1, ERT-2, ERT2, ERT 2, ERK-2, ERK2, ERK-1, ERK1, ERK 2, ERK 1, ATGA0191-10ug, ATGA0191-20ug, ATGA0191-50ug, ATGA0191-100ug, ATGA0191-250ug, ATGA0191-500ug, ATGA0191-1mg, ATGA0191-010, ATGA0191-020, ATGA0191-050, ATGA0191-100, ATGA0191-250, ATGA0191-500, ATGA0191-01M

The Recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: ERK2 recombinant protein
Lane 2.: ERK1 recombinant protein The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: ERK1(MAPK3) Transfected 293T lysate