Human 14-3-3 gamma antibody

Antibodies

Monoclonal Antibody

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, Beta, Gamma, Epsilon, Delta, Zeta, Tau and Eta that have been identified in mammals. The 14-3-3 gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Monoclonal

Signal Transduction

1-4-3-3g, 1433 gamma, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide, YWHAG, 14-3-3 gamma, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, gamma polypeptide 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP1, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide., ATGA0312-10ug, ATGA0312-20ug, ATGA0312-50ug, ATGA0312-100ug, ATGA0312-250ug, ATGA0312-500ug, ATGA0312-1mg, ATGA0312-10, ATGA0312-20, ATGA0312-50, ATGA0312-100, ATGA0312-250, ATGA0312-500, ATGA0312-1

The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human YWHAZ
Lane 2.: Recombinant Human YWHAB
Lane 3.: Recombinant Human YWHAE
Lane 4.: Recombinant Human YWHAH
Lane 5.: Recombinant Human YWHAG
Lane 6.: Recombinant Human SFN
Lane 7.: Recombinant Human YWHAQ The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: A431 cell lysate
Lane 3.: K562 cell lysate
Lane 4.: NIH3T3 cell lysate