Human Cytokeratin 20/KRT20 antibody

Antibodies

Monoclonal Antibody

Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Cancer

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, ATGA0337-10ug, ATGA0337-20ug, ATGA0337-50ug, ATGA0337-100ug, ATGA0337-250ug, ATGA0337-500ug, ATGA0337-1mg, ATGA0337-010, ATGA0337-020, ATGA0337-050, ATGA0337-100, ATGA0337-250, ATGA0337-500, ATGA0337-01M

The Recombinant protein (25ng) and Cell lysates of HeLa, A431(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT20 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human KRT20
Lane 2.: A431 cell lysate