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Human GRP94/HSP90B1 antibody

Human GRP94/HSP90B1 antibody

Item no.	NKM-ATGA0348-100ul
Manufacturer	NKMAX
Amount	100 ul
Quantity options	100 ul 50 ul
Category	Oncology Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Carcinoma Sarcoma Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, ICC, ELISA
Clone	AT94B9
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Conjugate/Tag	Unconjugated
Citations	Randow F., et al. (2001) Nat Cell Biol. 3(10): 231. Li Z., et al. (2002) Front Biosci. 7: 731-751.
NCBI	NP_003290
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human GRP94 (22-803aa) purified from E.coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Metabolism
Manufacturer - Search Terms	Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGA0348-10ug, ATGA0348-20ug, ATGA0348-50ug, ATGA0348-100ug, ATGA0348-250ug, ATGA0348-500ug, ATGA0348-1mg, ATGA0348-010, ATGA0348-020, ATGA0348-050, ATGA0348-100, ATGA0348-250, ATGA0348-500, ATGA0348-01M
Manufacturer - Alternative Names	Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein
Manufacturer - Similar Names	Tumor rejection antigen GP96, Tumor rejection antigen 1, TRA1, Stress inducible tumor rejection antigen GP96, HSP90B1, Heat shock protein 90 kDa beta member 1, GRP94, GRP 9, GRP9, GP96 homolog, GP96, Glucose regulated protein 94kDa, Endothelial cell (HBMEC) glycoprotein, Endoplasmin, ECGP, 94 kDa glucose-regulated protein, 94 kDa glucose regulated protein, 94kDa glucose regulated protein, 94kDa glucose-regulated protein, ATGA0348-10ug, ATGA0348-20ug, ATGA0348-50ug, ATGA0348-100ug, ATGA0348-250ug, ATGA0348-500ug, ATGA0348-1mg, ATGA0348-010, ATGA0348-020, ATGA0348-050, ATGA0348-100, ATGA0348-250, ATGA0348-500, ATGA0348-01M
IF Comment	ICC/IF analysis of GRP94 in U87MG cells. The cell was stained with ATGA0348 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The Recombinant protein (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GRP94 Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: HepG2 cell lysate
FACS Comment	Flow cytometry analysis of GRP94 in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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