Comparison

Human S100A4 antibody

Item no. NKM-ATGA0384-100ul
Manufacturer NKMAX
Amount 100 ul
Quantity options 100 ul 50 ul
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, ICC, ELISA
Clone AT1C3
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG2b Kappa
Conjugate/Tag Unconjugated
Citations Stoler A., et al. (1985) Proc Natl Acad Sci USA. 82(2): 570-574.
Choudhary C., et al. (2009) Science. 325: 834-840.
Bian Y., et al. (2014) J Proteomics. 96: 253-262.
NCBI NP_002952
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias S100 calcium binding protein A4,18A2,42a,Capl,FSp1,metastasin,Mts1,PeL98,pk9a
Available
Manufacturer - Type
Antibodies
Manufacturer - Applications
ELISA, WB, ICC/IF
Manufacturer - Category
Monoclonal Antibody
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description
S100 calcium-binding protein A4 (S100A4) is member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human S100A4 (1-101aa) purified from E. coli
Ab Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Clonality
Monoclonal
Ab Purification Comment
By protein-A affinity chromatography
Gene Category
Cardiovascular
Manufacturer - Search Terms
S100A4, S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a, ATGA0384-10ug, ATGA0384-20ug, ATGA0384-50ug, ATGA0384-100ug, ATGA0384-250ug, ATGA0384-500ug, ATGA0384-1mg, ATGA0384-10, ATGA0384-20, ATGA0384-50, ATGA0384-100, ATGA0384-250, ATGA0384-500, ATGA0384-1
Manufacturer - Similar Names
S100A4, S100 calcium binding protein A4, 18A2, 42a, Capl, FSp1, metastasin, Mts1, PeL98, pk9a, ATGA0384-10ug, ATGA0384-20ug, ATGA0384-50ug, ATGA0384-100ug, ATGA0384-250ug, ATGA0384-500ug, ATGA0384-1mg, ATGA0384-10, ATGA0384-20, ATGA0384-50, ATGA0384-100, ATGA0384-250, ATGA0384-500, ATGA0384-1
IF Comment
ICC/IF analysis of S100A4 in A549 cells. The cell was stained with ATGA0384 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of S100A4 in HeLa cells line, stained with DAPI (Blue) for nucleus staining, Wheat germ agglutinin (WGA) with fluorescein conjugate (Red) for plasma membrane staining and monoclonal anti-human S100A4 antibody{2004010323173395.jpg}
WB Comment
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : HeLa cell lysate
Lane 2. : A549 cell lysate
Lane 3. : NIH/3T3 cell lysate
Lane 4. : 3T3-L1 cell lysate

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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