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Human HO-1/HMOX1/HSP32 antibody

Human HO-1/HMOX1/HSP32 antibody

Item no.	NKM-ATGA0454-100ul
Manufacturer	NKMAX
Amount	100 ul
Quantity options	100 ul 50 ul
Category	Oncology Neuroscience Molecular Targets for Neuroscience Antibodies By Cell Type Hematology Leukemia Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, ICC, ELISA
Clone	AT1D6
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
Citations	Soares MP., et al. (2001) Immunol Rev. Vareille M., et al. (2008) J Immunol. 180(8): 5720-6.
NCBI	NP_002124
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	HO-1,Heat shock protein 32,HSP32,bK286B10,D8Wsu38e,Heme oxygenase (decycling) 1,Heme oxygenase 1,Hemox,Hmox,HMOX 1,HMOX1,HO,HO 1,HO1.
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, FACS, ICC/IF
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Heme oxygenase 1 belongs to the heme oxygenase family and is an essential enzyme in heme catabolism. It cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Also this protein is known to play an important role in the regulation of cardiovascular function and its adaptive response to a variety of stressors.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human Heme oxygenase1 (1-266aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Neuroscience
Manufacturer - Search Terms	HO1, HMOX1, HSP32, HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1., ATGA0454-10ug, ATGA0454-20ug, ATGA0454-50ug, ATGA0454-100ug, ATGA0454-250ug, ATGA0454-500ug, ATGA0454-1mg, ATGA0454-10, ATGA0454-20, ATGA0454-50, ATGA0454-100, ATGA0454-250, ATGA0454-500, ATGA0454-1
Manufacturer - Similar Names	HO1, HMOX1, HSP32, HO-1, Heat shock protein 32, HSP32, bK286B10, D8Wsu38e, Heme oxygenase (decycling) 1, Heme oxygenase 1, Hemox, Hmox, HMOX 1, HMOX1, HO, HO 1, HO1., ATGA0454-10ug, ATGA0454-20ug, ATGA0454-50ug, ATGA0454-100ug, ATGA0454-250ug, ATGA0454-500ug, ATGA0454-1mg, ATGA0454-10, ATGA0454-20, ATGA0454-50, ATGA0454-100, ATGA0454-250, ATGA0454-500, ATGA0454-1
IF Comment	ICC/IF analysis of HMOX in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF1b.jpg}ICC/IF analysis of HMOX in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HMOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) {ATGA0454-IF2a.jpg} {ATGA0454-IF2b.jpg}
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMOX antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: A549 cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: TF-1 cell lysate
FACS Comment	Flow cytometry analysis of HMOX in HeLa cells. The cell was stained with ATGA0454 at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 12/4/2025

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