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Human RAB7A antibody

Human RAB7A antibody

Item no.	NKM-ATGA0471-100ul
Manufacturer	NKMAX
Amount	100 ul
Quantity options	100 ul 50 ul
Category	Neuroscience Targets for Rodent Neurosciences Antibodies Cell Biology Cytoskeleton Flow Cytometry Western Blot ELISA Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry small GTPases Rab Subfamily
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, FC, ICC, ELISA
Clone	AT10E4
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
Citations	Feng Y., et al. (1995) J Cell Biol. 131: 1435-52. Barbero P., et al. (2002) J Cell Biol. 156: 511-8.
NCBI	NP_004628
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Ras-related protein Rab-7a,RAB7,RAB7A member RAS oncogene family,CMT2B,Charcot-Marie-Tooth neuropathy 2B
Similar products	Rab7a, ATGA0471-10ug, ATGA0471-20ug, ATGA0471-50ug, ATGA0471-100ug, ATGA0471-250ug, ATGA0471-500ug, ATGA0471-1mg, ATGA0471-10, ATGA0471-20, ATGA0471-50, ATGA0471-100, ATGA0471-250, ATGA0471-500, ATGA0471-1
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF, FACS
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles.
Concentration	1mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human RAB7A (1-207aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Tags & Cell Markers
Manufacturer - Search Terms	Rab7a, ATGA0471-10ug, ATGA0471-20ug, ATGA0471-50ug, ATGA0471-100ug, ATGA0471-250ug, ATGA0471-500ug, ATGA0471-1mg, ATGA0471-10, ATGA0471-20, ATGA0471-50, ATGA0471-100, ATGA0471-250, ATGA0471-500, ATGA0471-1
IF Comment	ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: MCF7 cell lysate Lane 4.: PC3 cell lysate Lane 5.: 293T cell lysate Lane 6.: SK-OV-3 cell lysate
FACS Comment	Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 12/4/2025

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