Human ATG3 antibody

Antibodies

Monoclonal Antibody

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Monoclonal

Ubiquitination

ATG3, ubiquitin-like-conjugating enzyme ATG3 isoform 1, APG3, APG3-LIKE, APG3L, PC3-96, ATGA0493-10ug, ATGA0493-20ug, ATGA0493-50ug, ATGA0493-100ug, ATGA0493-250ug, ATGA0493-500ug, ATGA0493-1mg, ATGA0493-10, ATGA0493-20, ATGA0493-50, ATGA0493-100, ATGA0493-250, ATGA0493-500, ATGA0493-1

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: ATG3 transfected 293T cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATG3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: Raji cell lysate
Lane 3.: Jurkat cell lysate