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Human beta-Actin antibody

Human beta-Actin antibody

Item no.	NKM-ATGA0570-500ul
Manufacturer	NKMAX
Amount	500 ul
Quantity options	100 ug 1 ml 500 ul 50 ug
Category	Neuroscience Neural Lineage Markers Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies Immunostaining Immunochemistry
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, ICC, ELISA
Clone	AT4G6
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	Unconjugated
Citations	Gunning PW., et al. (2015) Journal of Cell Science. 128(11): 2009-2019. Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163(4): 673-8. Yang YJ et al., Liquid Chromatography/Tandem Mass Spectrometry Analysis of Sophora flavescens Aiton and Protective Effects against Alcohol-Induced Liver Injury and Oxidative Stress in Mice. (Antioxidants (Basel). 2024) Yang YJ et al., Korean Mistletoe (Viscum album var. coloratum) Ethanol Extracts Enhance Intestinal Barrier Function and Alleviate Inflammation. (Antioxidants (Basel) 2025) Kim MJ et al., Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell. (Int. J. Mol. Sci. 2025) Han JY et al., Cytoplasmic HMGB1 promotes the activation of JAK2-STAT3 signaling and PD-L1 expression in breast cancer. (Mol Med. 2025) Kim MJ et al., Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition. (Sci Rep. 2025)
NCBI	NP_001092
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Actin,cytoplasmic 1,Beta-actin
Similar products	actin, beta actin, beta-actin, b-Actin, ATGA0457, ATGA0570-10ug, ATGA0570-20ug, ATGA0570-50ug, ATGA0570-100ug, ATGA0570-250ug, ATGA0570-500ug, ATGA0570-1mg, ATGA0570-10, ATGA0570-20, ATGA0570-50, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-1
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	ELISA, WB, ICC/IF
Manufacturer - Category	Monoclonal Antibody
Storage Conditions	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Description	Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Concentration	0.2mg/ml (determined by BCA assay)
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen	Recombinant human ACTB (1-375aa) purified from E. coli
Ab Usage	The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species	Human
Clonality	Monoclonal
Ab Purification Comment	By protein-A affinity chromatography
Gene Category	Cancer
Manufacturer - Search Terms	b-Actin, beta actin, beta-actin, actin, ATGA0457, ATGA0570-10ug, ATGA0570-20ug, ATGA0570-50ug, ATGA0570-100ug, ATGA0570-250ug, ATGA0570-500ug, ATGA0570-1mg, ATGA0570-10, ATGA0570-20, ATGA0570-50, ATGA0570-100, ATGA0570-250, ATGA0570-500, ATGA0570-1
IF Comment	ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
WB Comment	The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: PC3 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: U87-MG cell lysate Lane 7.: NIH-3T3 cell lysateThe mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysate Lane 3.: Spleen tissue lysate Lane 4.: Bone marrow tissue lysate Lane 5.: Stomach tissue lysate The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ACTB antibody 1:1000 Lane 2.: ACTB antibody 1:3000 Lane 3.: ACTB antibody 1:5000 Lane 4.: ACTB antibody 1:10000

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500 ul

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Delivery expected until 12/4/2025

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