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CD14 antibody

CD14 antibody

Item no.	OAEE00081
Manufacturer	AVIVA Systems Biology
Amount	0.1 mg (C100)
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Antibodies By Cell Type Hematology Leukemia Lymphoma Flow Cytometry Immunoprecipitation Western Blot ELISA Primary Antibodies Immunostaining Functional Assays
Type	Antibody Monoclonal
Applications	WB, FC, IP, ELISA, FA
Clone	MEM-18
Specific against	Human (Homo sapiens), Primate
Host	Mouse
Isotype	IgG1
Purity	> 95% (by SDS-PAGE)
Citations	Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol. 1986 Dec;16(12):1583-9. Bazil V, Baudys M, Hilgert I, Stefanova I, Low MG, Zbrozek J, Horejsi V: Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol. 1989 Jul;26(7):657-62. Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y: The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain. Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6. Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J: Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med. 2002 Oct 21;196(8):1017-24. Funda DP, TuckovÃ¡ L, FarrÃ© MA, Iwase T, Moro I, TlaskalovÃ¡-HogenovÃ¡ H: CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect Immun. 2001 Jun;69(6):3772-81. StÃ¶ckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33. Weiss TS, Lichtenauer M, Kirchner S, Stock P, Aurich H, Christ B, Brockhoff G, Kunz-Schughart LA, Jauch KW, Schlitt HJ, Thasler WE. Hepatic progenitor cells from adult human livers for cell transplantation. Gut. 2008 Aug;57(8):1129-38. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, SchÃ¼tz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov;19(11):1271-9. Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitismedia. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43. FernÃ¡ndez-Real JM, Broch M, Richart C, Vendrell J, LÃ³pez-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulinsensitivity. J Clin Endocrinol Metab. 2003 Apr;88(4):1780-4. Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83. Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	monocyte differentiation antigen CD14;myeloid cell-specific leucine-rich glycoprotein.
Shipping Condition	Cool pack
Available
Specificity	Human, Non-human Primate
Manufacturer - Type	Monoclonal Antibody
Manufacturer - Category	Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Products/Primary Antibodies
Shipping Temperature	Wet Ice
Gene symbol	CD14
Gene Fullname	CD14 molecule
Product format	Phosphate buffered saline (PBS), pH 7.4
Reconstitution and storage	2°C to 8°C
Description of target	Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Nucleotide accession_num	NM_000591.3; NM_001040021.2; NM_001174104.1; NM_001174105.1
Protein accession_num	NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1
Protein name	Monocyte differentiation antigen CD14
Clonality	Monoclonal
Purification	Purified
Immunogen	A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Manufacturer - Specificity	The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64. HLDA III; WS Code M 253 HLDA IV; WS Code M 314 HLDA V; WS Code M MA087 HLDA VI; WS Code M MA95
Formulation	Low endotoxin
Concentration	1 mg/ml
Storage Buffer	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg (C100)

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