Rabbit antibody to PGP 9.5 (50-100)

Function: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Subcellular location: Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note: About 30% of total UCHL1 is associated with membranes in brain.
Tissue specificity: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
Involvement in disease: Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5); also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.

IHC, WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

12 months after reconstitution

1. Day I.N.M, et al. Biochem. J. 268:521-524(1990)
2. Honore B, et al. FEBS Lett. 280:235-240(1991)

A synthetic peptide from aa region 50-100 of mouse PGP 9.5 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

http://www.uniprot.org/uniprot/P09936
http://www.uniprot.org/uniprot/Q9R0P9
http://www.uniprot.org/uniprot/Q00981

Polyclonal

Lyophilised. This product contains 0.02% benzalkonium chloride.