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DFF45 Antibody

DFF45 Antibody

Item no.	PRS-1141-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
NCBI	DFFA
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Available
Manufacturer - Applications	DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Shipping Temperature	blue ice or room temperature
Storage Conditions	DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	45 kDa
Manufacturer - Research Area	Apoptosis
Background	DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
Protein Gi #	4758148
Purification	DFF45 Antibody is Antibody is DEAE purified.
Positive Control 1	PRS-1201-0.1mg
Positive Control 2	PRS-17-001-1pack
Concentration	1 mg/ml
Buffer	DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
Immunogen	DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.
Clonality	Polyclonal
NCBI Official Name	DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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