Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Rat |
Format |
Liquid |
Applications |
WB, IHC |
Amount |
50 uL |
Host |
Rabbit |
Item no. |
PRS-15-271-50uL |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Cancer, Neuroscience |
Immunogen |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1). |
Applications |
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 |
Positive Control 1 |
293T |
Predicted Molecular Weight |
Observed: 147kDa |
Purification |
Affinity purification |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage Conditions |
Store at -20˚ C. Avoid freeze / thaw cycles. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SIN3A |
Ncbi Gene Id # |
25942 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SIN3A |
Ncbi Official Full Name |
SIN3 transcription regulator family member A |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q96ST3 |
Background |
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. |
1st Image Caption |
Western blot analysis of extracts of 293T cells, using SIN3A antibody (15-271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
2nd Image Caption |
Immunohistochemistry of paraffin-embedded rat liver using SIN3A antibody (15-271) at dilution of 1:100 (40x lens). |
3rd Image Caption |
Immunohistochemistry of paraffin-embedded human lung cancer using SIN3A antibody (15-271) at dilution of 1:100 (40x lens). |
4th Image Caption |
Immunohistochemistry of paraffin-embedded human liver cancer using SIN3A antibody (15-271) at dilution of 1:100 (40x lens). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.