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ADAM11 Antibody

ADAM11 Antibody

Manufacturer	ProSci
Category	Antibodies Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse, Rat
Format	Liquid
Applications	WB, IF
Amount	50 uL
Host	Rabbit
Item no.	PRS-15-449-50uL
eClass 6.1	32160702
eClass 9.0	32160702
Available
Shipping	blue ice or RT
By Research Area	Cancer, Cell Cycle, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 290-430 of human ADAM11 (NP_002381.2).
Applications	WB: 1:500 - 1:2000 IF: 1:50 - 1:200
Positive Control 1	U-87MG
Positive Control 2	Mouse brain
Predicted Molecular Weight	Observed: 83kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	ADAM11
Ncbi Gene Id #	4185
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	ADAM11
Ncbi Official Full Name	Disintegrin and metalloproteinase domain-containing protein 11
Ncbi Organism	Homo sapiens
Swissprot #	O75078
Background	This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. This gene represents a candidate tumor suppressor gene for human breast cancer based on its location within a minimal region of chromosome 17q21 previously defined by tumor deletion mapping. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
1st Image Caption	Western blot analysis of extracts of various cell lines, using ADAM11 antibody (15-449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
2nd Image Caption	Immunofluorescence analysis of C6 cells using ADAM11 antibody (15-449) at dilution of 1:100. Blue: DAPI for nuclear staining.
3rd Image Caption	Immunofluorescence analysis of NIH/3T3 cells using ADAM11 antibody (15-449) at dilution of 1:100. Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 6/6/2024