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GBP1 Antibody

GBP1 Antibody

Manufacturer	ProSci
Category	Antibodies Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Specific against	Human, Mouse, Rat
Format	Liquid
Applications	WB, IF, IHC
Amount	50 uL
Host	Rabbit
Item no.	PRS-19-195-50uL
eClass 6.1	32160702
eClass 9.0	32160702
Available
Shipping	blue ice or RT
By Research Area	Chemokines & Cytokines, Immunology, Innate Immunity, Signal Transduction
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 383-592 of human GBP1 (NP_002044.2).
Applications	WB: 1:500 - 1:2000 IHC: 1:20 - 1:200 IF: 1:10 - 1:100
Positive Control 1	5637
Positive Control 2	Mouse liver
Positive Control 3	Rat liver
Predicted Molecular Weight	Observed: 68kDa
Purification	Affinity purification
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage Conditions	Store at -20˚ C. Avoid freeze / thaw cycles.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	GBP1
Ncbi Gene Id #	2633
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	GBP1
Ncbi Official Full Name	guanylate binding protein 1, interferon-inducible
Ncbi Organism	Homo sapiens
Swissprot #	P32455
Background	Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3.
1st Image Caption	Western blot analysis of extracts of various cell lines, using GBP1 antibody (19-195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
2nd Image Caption	Immunohistochemistry of paraffin-embedded human liver cancer using GBP1 antibody (19-195) at dilution of 1:100 (40x lens).
3rd Image Caption	Immunofluorescence analysis of MCF7 cells using GBP1 antibody (19-195). Blue: DAPI for nuclear staining.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 uL

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Delivery expected until 6/6/2024