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CX3CR1 Antibody

CX3CR1 Antibody

Item no.	PRS-2093-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Neuroscience Neural Lineage Markers Neuroinflammation Antibodies Flow Cytometry Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, FC, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Raport et al. Gene 1995;163:295-9. Combadiere et al. DNA Cell Biol 1995;14:673-80. Combadiere et al. Biochem Biophys Res Commun 1998;253:728-32. Harrison et al. Neurosci Lett 1994;169:85-9. Lucas et al. Smooth muscle cells in human atherosclerotic plaques express the fractalkine receptor CX3CR1 and undergo chemotaxis to the CX3C chemokine fractalkine (CX3CL1). Circulation. 2003;108(20):2498-504.PMID: 14581400 Barlic et al. IL-15 and IL-2 oppositely regulate expression of the chemokine receptor CX3CR1. Blood. 2003;102(10):3494-503. PMID: 12881312 Yajima et al. Elevated levels of soluble fractalkine in active systemic lupus erythematosus: potential involvement in neuropsychiatric manifestations. Arthritis Rheum. 2005;52(6):1670-5. PMID: 15934075 Kumar et al. Role of CX3CR1 receptor in monocyte/macrophage driven neovascularization. PLoS One. 2013;8(2):e57230. PMID: 23437346 Beutner et al. Engineered stem cell-derived microglia as therapeutic vehicle for experimental autoimmune encephalomyelitis. Gene Ther. 2013;20(8):797-806. PMID: 23324824 Wang et al. TGF-β/Smad3 signalling regulates the transition of bone marrow-derived macrophages into myofibroblasts during tissue fibrosis. Oncotarget. 2016;7(8):8809-22. PMID: 26684242 Kang et al. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via αvβ1 integrin. J Immunol. 2012;188(3):1108-16. PMID: 22198947 Umemura et al. Tumor-infiltrating myeloid-derived suppressor cells are pleiotropic-inflamed monocytes/macrophages that bear M1- and M2-type characteristics. J Leukoc Biol. 2008;83(5):1136-44. PMID: 18285406 You et al. Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis. Invest Ophthalmol Vis Sci. 2007;48(11):5290-8. PMID: 17962485 Matsunawa et al. Elevated serum levels of soluble CX3CL1 in patients with microscopic polyangiitis. Clin Exp Rheumatol. 2009;27(1):72-8. PMID: 19327232 Sato et al. Involvement of CX3CL1/CX3CR1 axis in etanercept therapy for patients with active rheumatoid arthritis. Open Access Rheumatol. 2011;3:1-7. PMID: 27789999 Clara Beutner. Microglia derived from embryonic stem cells and its application in CNS diseases. PhD Thesis. University of Bonn, Germany. 2012.PMID: Kristin Roy. Establishment of microglial precursors derived from human induced pluripotent stem cells to model SOD1-mediated amyotrophic lateral sclerosis. PhD Thesis. University of Bonn, Germany. 2012.PMID: Isabella Napoli. Establishment of Embryonic Stem Cell Derived Microglial Precursors and Application in an Animal Model of Alzheimer's Disease. PhD Thesis. University of Bonn, Germany. 2008.PMID: Kalaimathi Govindarajan. Role of KLF4 in regulation of myocardin induced SMC differentiation in human smooth muscle stem progenitor cells (hSMSPC). PhD Thesis. University of College Cork, Ireland. 2013.PMID: Rashmi Bankoti. Further characterization of pathogenic lung Th2 memory cells in experimental allergic asthma. PhD Thesis. University of Vienna, Austria. 2008.PMID:
NCBI	CX3CR1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CX3CR1 Antibody: V28,CCRL1,GPR13,CMKDR1,GPRV28,CMKBRL1,CX3C chemokine receptor 1,Beta chemokine receptor-like 1,C-X3-C CKR-1
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	CX3CR1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 40-44 kD Observed: 50 kD
Manufacturer - Research Area	Chemokines & Cytokines, Cancer, Infectious Disease
Background	CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
By Type	Chemokines & Cytokines, Cancer, Infectious Disease
Badges	Trial Size, KD, Most Cited, Most Images
Modifications	None
Protein Gi #	4503171
Purification	CX3CR1 Antibody is affinity chromatography purified via peptide column.
Isoforms	Human CX3CR1 has 3 isoforms, including isoform 1 (355aa, 40kD), isoform 2 (387aa, 44kD), isoform 3 (362aa, 41kD). Mouse CX3CR1 has one isoform (354aa, 40kD) and rat CX3CR1 also has one isoform (354aa, 40kD). 2093 can detect all isoforms of human, mouse and rat.
Validation	KD Validation (Figure 2): Anti-CX3CR1 antibody (2093) specificity was further verified by CX3CR1 specific knockdown. CX3CR1 signal in 293 cells transfected with CX3CR1 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. Regulated expression validation (Figure 13): CX3CR1 expression detected by anit-CX3CR1 antibodies (2093) was up-regulated by IL-2, which was inhibited by IL-15. Deficiency Validation (Figure 15): CX3CR1+ cells detected by anti-CX3CR1 antibodies is localized in vascular wall of CX3CR1⁺^/gfp mice, but not in CX3CR1^gfp/gfpmice, where cells remained in perivascular region.
Positive Control 1	PRS-1306-0.1mg
Positive Control 2	PRS-10-501-4um
Positive Control 3	PRS-1466-0.1mg
Positive Control 4	PRS-1208-0.1mg
Concentration	1 mg/mL
Buffer	CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	WB: 1 -2 μ g/mL; IF: 10-20 μ g/mL, IHC-P: 2 μ g/mL: Flow: 0.1 μ g/mL . Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples; Immunofluorescence in human, mouse and rat samples; Flow cytometry in human samples. All other applications and species not yet tested.
Homology	Predicted species reactivity based on immunogen sequence: Bovine: (82%)
Immunogen	Anti-CX3CR1 antibody (2093) was raised against a peptide corresponding to 20 amino acids near the amino terminus of mature human CX3CR1. The immunogen is located within the first 50 amino acids of CX3CR1.
Clonality	Polyclonal
NCBI Official Name	chemokine (C-X3-C motif) receptor 1
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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