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Nephrin Antibody

Nephrin Antibody

Item no.	PRS-2265-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Chen et al. Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage. FEBS J. 2017;284(7):1070-1081. PMID: 28194887 Yun et al. Expression of nephrin in the human placenta and fetal membranes. Mol Med Rep. 2015;12(4):5116-20.PMID: 26151763 Li et al. Mesenchymal stem cells protect podocytes from apoptosis induced by high glucose via secretion of epithelial growth factor. Stem Cell Res Ther. 2013;4(5):103. PMID: 24004644 Soetikno et al. Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats. J Nutr Biochem. 2013;24(5):796-802. PMID: 22898567 Piwkowska et al. Insulin increases glomerular filtration barrier permeability through dimerization of protein kinase G type Iα subunits. Biochim Biophys Acta. 2013;1832(6):791-804.PMID: 23454089 Toyama et al. Proteinuria in AMPD2-deficient mice. Genes Cells. 2012;17(1):28-38. PMID: 22212473 Huang et al. Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement. Int Urol Nephrol. 2019;51(2):359-367. PMID: 30536192 Piwkowska et al. Hydrogen peroxide induces dimerization of protein kinase G type Iα subunits and increases albumin permeability in cultured rat podocytes. J Cell Physiol. 2012;227(3):1004-16. PMID: 21520075 Rogacka et al. Metformin overcomes high glucose-induced insulin resistance of podocytes by pleiotropic effects on SIRT1 and AMPK. Biochim Biophys Acta Mol Basis Dis. 2018;1864(1):115-125. PMID: 29032153 Kestila et al. Mol. Cell 1998; 1:575-582. Tryggvason. J. Am. Soc. Nephrol. 1999; 10:2440-5. Tryggvason and Wartiovaara. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.
NCBI	NPHS1
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor
Available
Manufacturer - Applications	WB: 1-2 μg/mL; IHC: 1-5 μg/mL; IF: 10-20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Shipping Temperature	blue ice or room temperature
Storage Conditions	Nephrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Manufacturer - Research Area	Homeostasis
Background	Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
Protein Gi #	4758822
Purification	Nephrin Antibody is affinity chromatography purified via peptide column.
Isoforms	Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD). Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD). Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD). 2265 can detect isoforms of human, mouse and rat.
Positive Control 1	PRS-1405-0.1mg
Positive Control 2	PRS-1203-0.1mg
Positive Control 3	PRS-1285-0.1mg
Concentration	1 mg/mL
Buffer	Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
Immunogen	Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.
Clonality	Polyclonal
NCBI Official Name	nephrosis 1, congenital, Finnish type (nephrin)
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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