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ASC Antibody

ASC Antibody

Item no.	PRS-2287-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Oncology Antibodies Immunohistochemistry Western Blot ELISA Immunocytochemistry Primary Antibodies By Organ Breast Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, ICC, ELISA, IHC-P
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Masumoto et al. J Biol Chem. 1999;274(48):33835-8. Masumoto et al. Exp Cell Res. 2001;262(2):128-133. Conway et al. Cancer Res. 2000;60(22):6236-42 McConnell et al. Cancer Res. 2000;60(22):6243-7. (WD0102) Dowds et al. Cryopyrin-induced interleukin 1beta secretion in monocytic cells: enhanced activity of disease-associated mutants and requirement for ASC. J Biol Chem. 2004;279(21):21924-8. PMID: 15020601
NCBI	PYCARD
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ASC Antibody: ASC,TMS,TMS1,CARD5,TMS-1,ASC,Apoptosis-associated speck-like protein containing a CARD,Caspase recruitment domain-containing protein 5,hASC
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	ASC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 22kD Observed: 22 kD
Manufacturer - Research Area	Apoptosis
Background	ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
By Type	Apoptosis, Inflammasomes
Badges	Trial Size, IAV, KD, Most Cited
Modifications	None
Protein Gi #	10801602
Purification	ASC Antibody is affinity chromatography purified via peptide column.
Isoforms	Human ASC has 3 isoforms, including isoform 1 (195aa, 22kD), isoform 2 (176aa, 20kD), and isoform 3 (135aa, 15kD). Mouse ASC has one isoform (193aa, 22kD) and rat ASC also has one isoform (193aa, 22kD). 2287 can detect all human isoforms.
Validation	Independent Antibody Validation in Cell lines (Figure 2) shows similar ASC expression profile in human and mouse cell lines detected by two independent anti-ASC antibodies that recognize different epitopes, 2287 against C-terminus domain and 39-001 against full length human recombinant protein. ASC proteins are detected in some of tested cell lines at different expression levels by the two independent antibodies.
Positive Control 1	PRS-1209-0.1mg
Positive Control 2	PRS-17-009-1pack
Positive Control 3	PRS-10-901-4um
Positive Control 4	PRS-1208-0.1mg
Concentration	1 mg/mL
Buffer	ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	WB: 1-2 μ g/mL; ICC: 5 μ g/mL; IHC-P: 2.5 μ g/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Homology	Predicted species reactivity based on immunogen sequence: Mouse (57%), Rat (57%)
Immunogen	Anti-ASC antibody (2287) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ASC. The immunogen is located within the last 50 amino acids of ASC.
Clonality	Polyclonal
NCBI Official Name	PYD and CARD domain containing
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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