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Smac Antibody

Smac Antibody

Item no.	PRS-2409-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Quantity options	0.02 mg 0.1 mg
Category	Oncology Antibodies Immunohistochemistry Immunoprecipitation Western Blot ELISA Primary Antibodies By Organ Breast Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, IP, ELISA, IHC-P
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Citations	Du et al. Cell 2000; 102:33-42. Verhagen et al. Cell 2000;102:43-53. Srinivasula et al. J. Biol. Chem. 2000; 275:36152-7. Chai et al. Nature 2000; 406:855-62. Ho et al. XIAP activity dictates Apaf-1 dependency for caspase 9 activation. Mol Cell Biol. 2007;27(16):5673-85. PMID: 17562856 Kim et al. Expression of apoptosis-related proteins and its clinical implication in surgically resected gastric carcinoma. Virchows Arch. 2011;459(5):503-10. PMID: 21947931 Saumet et al. Type 3 repeat/C-terminal domain of thrombospondin-1 triggers caspase-independent cell death through CD47/alphavbeta3 in promyelocytic leukemia NB4 cells. Blood. 2005;106(2):658-67. PMID: 15784731 Genestier et al. Staphylococcus aureus Panton-Valentine leukocidin directly targets mitochondria and induces Bax-independent apoptosis of human neutrophils. J Clin Invest. 2005;115(11):3117-27. PMID: 16276417 Flygare et al. Discovery of a potent small-molecule antagonist of inhibitor of apoptosis (IAP) proteins and clinical candidate for the treatment of cancer(GDC-0152). J Med Chem. 2012;55(9):4101-13. PMID: 22413863 Dohi et al. Mitochondrial survivin inhibits apoptosis and promotes tumorigenesis. J Clin Invest. 2004;114(8):1117-27. PMID: 15489959 Watanabe et al. MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline. Dev Cell. 2011;20(3):364-75. PMID: 21397847 Xia et al. Tumor cell dependence on Ran-GTP-directed mitosis. Cancer Res. 2008;68(6):1826-33. PMID: 18339863 Otera et al. Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling. J Cell Biol. 2016;212(5):531-44. PMID: 26903540 Lucken-Ardjomande et al. Contributions to Bax insertion and oligomerization of lipids of the mitochondrial outer membrane. Cell Death Differ. 2008;15(5):929-37. PMID: 18259190 Lucken-Ardjomande et al. Bax activation and stress-induced apoptosis delayed by the accumulation of cholesterol in mitochondrial membranes. Cell Death Differ. 2008;15(3):484-93.PMID: 18084240 Sedelies et al. Blocking granule-mediated death by primary human NK cells requires both protection of mitochondria and inhibition of caspase activity. Cell Death Differ. 2008;15(4):708-17. PMID: 18202705 Waterhouse et al. Functional dissociation of DeltaPsim and cytochrome c release defines the contribution of mitochondria upstream of caspase activation during granzyme B-induced apoptosis. Cell Death Differ. 2006;13(4):607-18. PMID: 16167065 Becatti et al. The involvement of Smac/DIABLO, p53, NF-kB, and MAPK pathways in apoptosis of keratinocytes from perilesional vitiligo skin: Protective effects of curcumin and capsaicin. Antioxid Redox Signal. 2010;13(9):1309-21. PMID: 20085492 Moubarak et al. FAIM-L is an IAP-binding protein that inhibits XIAP ubiquitinylation and protects from Fas-induced apoptosis. J Neurosci. 2013;33(49):19262-75. PMID: 24305822 Ndubaku et al. Antagonism of c-IAP and XIAP proteins is required for efficient induction of cell death by small-molecule IAP antagonists. ACS Chem Biol. 2009;4(7):557-66.PMID: 19492850 Stichel et al. Mono- and double-mutant mouse models of Parkinson's disease display severe mitochondrial damage. Hum Mol Genet. 2007;16(20):2377-93. PMID: 17412759 Sun et al. Proteasome inhibitor-induced apoptosis is mediated by positive feedback amplification of PKCdelta proteolytic activation and mitochondrial translocation. J Cell Mol Med. 2008;12(6A):2467-81. PMID: 18298651 Martin-Latil et al. Bax is activated during rotavirus-induced apoptosis through the mitochondrial pathway. J Virol. 2007;81(9):4457-64. PMID: 17301139 Mayorga et al. Bcl-2 is a key factor for cardiac fibroblast resistance to programmed cell death. J Biol Chem. 2004;279(33):34882-9. PMID: 15184368
NCBI	Diablo
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Smac Antibody: Smac,AU040403,0610041G12Rik,1700006L01Rik,Smac,Diablo homolog,mitochondrial,Direct IAP-binding protein with low pI
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibodies
Shipping Temperature	Blue Ice
Storage Conditions	Smac antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight	Predicted: 27kD Observed: 20 kD
Manufacturer - Research Area	Apoptosis, Cancer
Background	Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
By Type	Apoptosis, Cancer
Badges	Trial Size, IAV, Most Cited, Most Images
Modifications	None
Protein Gi #	8953908
Purification	Smac Antibody is DEAE purified.
Isoforms	Human Smac has 3 isoforms, including isoform 1 (239aa, 27.1kD), isoform 2 (186aa, 21.2kD), and isoform 3 (195aa, 22.3kD). Mouse Smac has one isoform (237aa, 26.8kD) and Rat Smac also has one isoform (237aa, 26.9kD). 2409 can detect human, mouse and rat Smac.
Validation	Independent Antibody Validation in Cell lines (Figure 2) shows similar Smac expression profile in human and mouse cell lines detected by two independent anti-Smac antibodies that recognize different epitopes, 2409 against human C-terminus domain and 2411 against murine C-terminus domain. Smac proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. KO Validation (Figure 6): Anti-Smac antibody specificity was further verified by Smac knockout mice. Smac signal smac KO mice was disrupted as compared to control. Induced Expression Validation (Figure 6, 9): Smac expression detected by anit-Smac antibodies was up-regulated by STS or rPVL treatment. Overexpression Validation (Figure 10): Smac overexpression was detected by anit-Smac antibodies on smac transfected HEK293T cells.
Positive Control 1	PRS-1301-0.1mg
Positive Control 2	PRS-1316-0.1mg
Positive Control 3	PRS-11-201-4um
Concentration	1 mg/mL
Buffer	Smac Antibody is supplied in PBS containing 0.02% sodium azide.
Application Note	WB: 1 μ g/mL; IHC: 5 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Immunogen	Anti-Smac antibody (2409) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac.
Clonality	Polyclonal
NCBI Official Name	diablo homolog (Drosophila)
NCBI Organism	Homo sapiens
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
User Note	Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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