Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Canine |
Format |
Liquid |
Applications |
WB, ELISA |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
PRS-26-093-100ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction , Immunology |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GUCY1B3. |
Applications |
GUCY1B3 antibody can be used for detection of GUCY1B3 by ELISA at 1:12500. GUCY1B3 antibody can be used for detection of GUCY1B3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
293T Cell Lysate |
Predicted Molecular Weight |
70 kDa |
Purification |
Antibody is purified by peptide affinity chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GUCY1B3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
GUCY1B3 |
Accession # |
NP_000848 |
Protein Gi # |
4504215 |
Ncbi Gene Id # |
2983 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
GUCY1B3 |
Ncbi Official Full Name |
guanylate cyclase 1, soluble, beta 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q02153 |
Background |
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit and a beta subunit, typically GUCY1B3, catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide (NO) and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]).Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit and a beta subunit, typically GUCY1B3, catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide (NO) and nitrovasodilator drugs (Zabel et al., 1998 [PubMed 9742212]). |
Background References 1 |
Slavov, D., (2006) Int. J. Impot. Res. 18 (5), 432-437. |
1st Image Caption |
Antibody used in WB on Human 293T at 0.2-1 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.