Manufacturer |
ProSci
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat, Canine, Carp |
Format |
Liquid |
Applications |
WB, ELISA |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
PRS-28-162-100ul |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Transcription |
Immunogen |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MID1. |
Applications |
MID1 antibody can be used for detection of MID1 by ELISA at 1:312500. MID1 antibody can be used for detection of MID1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000. |
Positive Control 1 |
Cat. No. 1205 - Jurkat Cell Lysate |
Predicted Molecular Weight |
75 kDa, 75 kDa, 62 kDa |
Purification |
Antibody is purified by protein A chromatography method. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Buffer |
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Concentration |
batch dependent |
Storage Conditions |
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MID1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
MID1 |
Accession # |
NP_000372 |
Protein Gi # |
4557753 |
Ncbi Gene Id # |
4281 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
MID1 |
Ncbi Official Full Name |
midline 1 (Opitz/BBB syndrome) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
O15344 |
Background |
MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Several different transcript variants are generated by alternate splicing; however, the full length nature of two variants has not been determined. |
Background References 1 |
Winter, J., et al., (2004) Hum. Genet. 114 (6), 541-552. |
1st Image Caption |
Antibody used in WB on Human Jurkat 2.5 ug/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.