IRAK-4 Antibody

Independent Antibody Validation in Cell lines (Figure 2) shows similar IRAK4 expression profile in human cell lines detected by two independent anti-IRAK4 antibodies that recognize different epitopes, 3125 against C-terminus domain and 24-025 against recombinant fusion protein. IRAK4 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

KO validation (Figure 6): Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockout and mutant mice. IRAK4 signal in the macrophages of IRAK KO mice and mutant mice was disrupted as compared to wild type mice.

Regulated expression validation (Figure 7): IRAK4 expression detected by anit-IRAK4 antibodies (3125) was reduced with CSK-4 treatment, which was rescued by proteasome inhibitors.

KD validation (Figure 8): Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockdown. IRAK4 signal was disrupted in HEK293T cells transfected with IRAK4 siRNAs.